The Purification and Properties of a Uridine Diphosphate Glucose: Aldehyde Cyanohydrin β-Glucosyltransferase from Sorghum Seedlings
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چکیده
منابع مشابه
The biosynthesis of cyanogenic glycosides in higher plants. I. Purification and properties of a uridine diphosphate-glucose-ketone cyanohydrin beta-glucosyltransferase from Linum usitatissimum L.
An enzyme which catalyzes the transfer of glucose from UDP-glucose to a-hydroxyisobutyronitrile (acetone cyanohydrin) has been isolated from flax seedlings (Zinum usifafissimum L). The product of this reaction was shown to be identical with linamarin, one of the two cyanogenic glucosides produced by the flax plant. The partially purified UDP-glucose-ketone cyanohydrm /3-glucosyltransferase exhi...
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A salicylic acid (SA)-inducible uridine 5'-diphosphate (UDP)-glucose:SA 3-O-glucosyltransferase was extracted from oat (Avena sativa L. cv Dal) roots. Reverse phase high-performance liquid chromatography or anion exchange chromatography was used to separate SA from the product, beta-O-d-glucosylsalicylic acid. The soluble enzyme was purified 176-fold with 5% recovery using a combination of pH f...
متن کاملCrystallization and properties of uridine diphosphate glucose pyrophosphorylase from liver.
The purification and crystallization of uridine diphosphate glucose pyrophosphorylase from calf liver has been described. As much as 0.3 % of the extractable protein is the pyrophosphorylase, which catalyzes the synthesis of uridine diphosphate glucose. After recrystallizing the enzyme twice, constant specific activity is attained; it appears to be almost homogeneous on polyacrylamide gel elect...
متن کاملPurification and properties of the adenosine diphosphate-glucose and uridine diphosphate-glucose pyrophosphorylases of Mycobacterium smegmatis: inhibition and activation of the adenosine diphosphate-glucose pyrophosphorylase.
Crude extracts of Mycobacterium smegmatis catalyzed the synthesis of adenosine diphosphate-glucose (ADP-Glc), cytidine diphosphate-glucose, guanosine diphosphate-glucose (GDP-Glc), thymidine diphosphate-glucose (TDP-Glc), and uridine diphosphate-glucose (UDP-Glc). In these crude enzyme fractions, high concentrations of trehalose-P inhibited the ADP-Glc and GDP-Glc pyrophosphorylases but did not...
متن کاملIsolation of uridine diphosphate N-acetylglucosamine and uridine diphosphate glucuronic acid from mung bean seedlings.
It has been shown previously (1) that mung bean seedlings (Phaseolus aureus) contain uridine diphosphate derivatives of n-glucose, n-galactose, n-xylose, and L-arabinose. These seedlings, as well as a number of other higher plants, also contain enzymatic systems capable of catalyzing the reversible formation of sugar nucleotides from UTPI and various monosaccharide l-phosphates (2, 3) according...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79891-1